ANDERSON BASAL SALT MIXTUREANDERSON BASAL SALT MIXTURE
Product ID: A267
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY ANDERSON (1978, 1980)
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
Product ID: A267
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY ANDERSON (1978, 1980)
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
A267 Anderson Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 1.89 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Anderson (1978,
1980)
pH = 3.25 – 4.25
Application Notes
Plant Tissue Culture Tested
Plant Species: Rhododendron, Rubus (red and black raspberry).
Anderson achieved a two-fold increase in multiplication of red raspberries using this formulation
compared to MS. The optimal concentrations of growth regulators for shoot multiplication of red
and black raspberries was 0.1 – 2.5 μM IBA and 4.5 – 9.0 μM BA.
Anderson’s medium contains approximately ¼ strength NH4NO3 and KNO3 compared to MS.
References
Anderson, WC. 1978. Tissue culture propagation of Rhododendrons. In Vitro 14: 334.
Anderson, WC. 1980. Tissue culture propagation of red and black raspberries ,Rubus idaeus
and R. occidentalis . Acta Hort. 112: 13-20.
BANANA AGS BASAL MEDIUM
BANANA AGS BASAL MEDIUM
Product ID: B144
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, VITAMINS, AND PLANT GROWTH REGULATORS REQUIRED TO CULTURE BANANAS.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
CAPE SUNDEW/ VENUS FLYTRAP MULTIPLICATION BASAL MEDIUM
CAPE SUNDEW/ VENUS FLYTRAP MULTIPLICATION BASAL MEDIUM
Product ID: C206
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- & MICRONUTRIENTS, VITAMINS, & PGR'S REQUIRED FOR CARNIVOROUS PLANTS.
PLANT TISSUE CULTURE TESTED
C206
Cape Sundew/ Venus Flytrap Multiplication Basal Medium
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
2.12 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients, vitamins, and plant growth regulators required to stimulate multiplication in cultures of Cape Sundew and Venus Flytrap.
Typical (unadjusted ) pH = 3.0 – 4.0; see certificate of analysis for lot-specific value. Adjust pH to desired level during medium preparation.
Application Notes
Plant Tissue Culture Tested
Plant Species: Cape Sundew (Drosera capensis), Venus Flytrap (Dionaea muscipula)
Revised 3/2010
CARNIVOROUS PLANT (NEPENTHES) BASAL SALT MIXTURE
Application Notes
Plant Tissue Culture Tested
Plant species: Carrot (Daucus carota )
References
Establishment and Maintenance of Carrot Callus
Carrot [Daucus carota L. subsp. Sativus (Hoffm.)] is a cool season plant grown for its edible storage taproot. Since the discovery of somatic embryogenesis in carrot culture was made by Steward and Reinert, it has become a model system for investigating many biochemical, physiological, and genetic aspects of plant cell culture. This procedure will outline the method for establishing carrot callus cultures.
Materials Required
1. 10 glass or plastic petri dishes (100 mm in diameter)
2. 2 pairs of forceps and 2 scalpels
3. Sterile #2 cork borer and glass rod that fits it
4. 1000-ml beaker and 250-ml beaker
5. Waterproof marking pen and labels
6. 4 plastic slant racks to hold solid culture tubes
7. Analytical balance
8. Bunsen or ethanol burner
9. 500 ml of 20% Clorox solution supplemented with a few drops of Tween-20
10. 500 ml of sterile distilled water
11. 200 ml of 95% ethanol
12. Carrot Callus Initiation Medium, Product Number C212
13. Carrot Shoot Development Medium, Product Number C222
14. 1 or 2 healthy, undamaged, and regularly shaped carrot roots.
Procedures
1. Wipe down and turn on laminar flow hood 15 min before doing any work in the hood. Flame-sterilize instruments.
2. Clean carrot root by scrubbing under running tap water to remove any surface soil. Trim the carrot into 100-mm sections and place them in a 1000-ml beaker. Cover with 20% Clorox solution for approximately 20 min and then decant the Clorox solution. Rinse the explant three times in sterile distilled water covering the tissue with each rinse.
3. While carrot sections are being sterilized, prepare and dispense media into culture vessels according to protocols in the media preparation section. Place the culture vessels in racks and label them with respective treatments, medium, and date.
4. Transfer sterilized carrot slices to a sterile petri dish. Using a sterile cork borer, punch out 8-10 cylinders of tissue from the secondary phloem cambial region of the carrot slices. Do not punch out more than two cylinders of tissue at one time, as you may have difficulty in removing them from the cork borer. Using a sterile glass rod, push the cylinders from the cork borer into a sterile petri dish. Using a sharp, sterile scalpel, remove the ends of the tissue cylinder. Next cut the remaining portion of the cylinder into 2- to 3-mm-thick sections. Using a sterile petri plate on the balance, weigh each explant section individually and inoculate into the culture vessels containing the Carrot Callus Initiation Medium, placing one explant per tube. Repeat this procedure until all culture vessels containing the Carrot Callus Initiation medium have been inoculated. Use a different forcep for each explant and flame the forceps between transfers. Flame the lid of the culture tubes immediately after opening and before closing.
Callus should be removed from the primary explant after 45 days. The calli can be subcultured onto the same medium for further callus growth or it can be subcultured onto the Carrot Shoot Development medium for shoot initiation.
CARROT SHOOT DEVELOPMENT BASAL MEDIUM
CARROT SHOOT DEVELOPMENT BASAL MEDIUM
Product ID: C222
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, VITAMINS, AND PGR'S REQUIRED TO INITIATE SHOOTS FROM CARROT CALLUS.
PLANT TISSUE CULTURE TESTED
C222 Carrot Shoot Development Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.21 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and plant growth
regulators required to initiate shoots from carrot callus.
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture Tested
Plant species: Carrot (Daucus carota )
References
CHEE & POOL C2d VITIS BASAL MEDIUM
CHEE & POOL C2d VITIS BASAL MEDIUM
Product ID: C287
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY CHEE & POOL (1987).
PLANT TISSUE CULTURE TESTED
C287
Chee & Pool C2d Vitis
Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 4.49 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chee
and Pool (1987).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture Tested
Plant Species: Grape (Vitis spp)
This medium was developed to improve grape shoot multiplication. This formulation has
reduced levels of chloride, Iodide, and manganese compared to MS. Calcium chloride was
replaced by calcium nitrate, thus improving the quality of grave shoots.
References
Chee, R and RM Pool. 1987. Improved inorganic media constituents for in vitro shoot
multiplication of Vitis. Sci. Hort. 32: 85.
CHU N6 VITAMIN SOLUTION (1000x)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 1.89 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Anderson (1978,
1980)
pH = 3.25 – 4.25
Application NotesPlant Tissue Culture Tested
Plant Species: Rhododendron, Rubus (red and black raspberry).
Anderson achieved a two-fold increase in multiplication of red raspberries using this formulation
compared to MS. The optimal concentrations of growth regulators for shoot multiplication of red
and black raspberries was 0.1 – 2.5 μM IBA and 4.5 – 9.0 μM BA.
Anderson’s medium contains approximately ¼ strength NH4NO3 and KNO3 compared to MS.
References
Anderson, WC. 1978. Tissue culture propagation of Rhododendrons. In Vitro 14: 334.
Anderson, WC. 1980. Tissue culture propagation of red and black raspberries ,Rubus idaeus
and R. occidentalis . Acta Hort. 112: 13-20.
BANANA AGS BASAL MEDIUMBANANA AGS BASAL MEDIUM
Product ID: B144
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, VITAMINS, AND PLANT GROWTH REGULATORS REQUIRED TO CULTURE BANANAS.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
CAPE SUNDEW/ VENUS FLYTRAP MULTIPLICATION BASAL MEDIUMCAPE SUNDEW/ VENUS FLYTRAP MULTIPLICATION BASAL MEDIUM
Product ID: C206
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- & MICRONUTRIENTS, VITAMINS, & PGR'S REQUIRED FOR CARNIVOROUS PLANTS.
PLANT TISSUE CULTURE TESTED
C206
Cape Sundew/ Venus Flytrap Multiplication Basal Medium
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
2.12 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients, vitamins, and plant growth regulators required to stimulate multiplication in cultures of Cape Sundew and Venus Flytrap.
Typical (unadjusted ) pH = 3.0 – 4.0; see certificate of analysis for lot-specific value. Adjust pH to desired level during medium preparation.
Application Notes
Plant Tissue Culture Tested
Plant Species: Cape Sundew (Drosera capensis), Venus Flytrap (Dionaea muscipula)
Revised 3/2010
CARNIVOROUS PLANT (NEPENTHES) BASAL SALT MIXTURECARNIVOROUS PLANT (NEPENTHES) BASAL SALT MIXTURE
Product ID: C1935
Storage Temperature: 2 to 6 C
Description: CARNIVOROUS PLANT (NEPENTHES) BASAL SALT MIXTURE
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
C1935
Carnivorous Plant (Nepenthes) Basal Salt Mixture Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Carnivorous Plant Culture
Solubility:
Soluble in Water
Typical Working Concentration:
0.65 g/L
Storage Temp:
2 - 6° C
Storage Temp of Stock Solution:
Preparation of concentrated stock solution is not recommended as insoluble precipitates may form.
Other Notes:
Does not contain sucrose, plant growth regulators, or gelling agent.
Product ID: C1935
Storage Temperature: 2 to 6 C
Description: CARNIVOROUS PLANT (NEPENTHES) BASAL SALT MIXTURE
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
C1935
Carnivorous Plant (Nepenthes) Basal Salt Mixture Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Carnivorous Plant Culture
Solubility:
Soluble in Water
Typical Working Concentration:
0.65 g/L
Storage Temp:
2 - 6° C
Storage Temp of Stock Solution:
Preparation of concentrated stock solution is not recommended as insoluble precipitates may form.
Other Notes:
Does not contain sucrose, plant growth regulators, or gelling agent.
Application Notes
Plant Tissue Culture Tested
Plant Species: Carnivorous Plants
This medium is commonly supplemented with MS Vitamins (e.g., M533, M553) and 25 g/L sucrose.
References
R. de Fossard. Posted on the Plant TC Listserv, Sept. 2009. Subject: Nepenthes diatas. (http://lists.umn.edu/cgi-bin/wa?A2=ind0901&L=PLANT-TC&T=0&F=&S=&X=1050D3258E48269160&P=2728). Also posted on: http://www.labflytrap.com/forum/viewtopic.php?f=7&t=209&start=0.
CARROT CALLUS INTIATION MEDIUM FROM PITH TISSUE.
CARROT CALLUS INTIATION MEDIUM FROM PITH TISSUE.
Product ID: C212
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, VITAMINS, & PLANT GROWTH REGULATORS REQUIRED TO INITIATE CARROT CALLUS
PLANT TISSUE CULTURE TESTED
C212
Carrot Callus Initiation
Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.21 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and plant growth
regulators required to initiate carrot callus from root tissue.
pH = 3.5 – 4.5
Plant Tissue Culture Tested
Plant Species: Carnivorous Plants
This medium is commonly supplemented with MS Vitamins (e.g., M533, M553) and 25 g/L sucrose.
References
R. de Fossard. Posted on the Plant TC Listserv, Sept. 2009. Subject: Nepenthes diatas. (http://lists.umn.edu/cgi-bin/wa?A2=ind0901&L=PLANT-TC&T=0&F=&S=&X=1050D3258E48269160&P=2728). Also posted on: http://www.labflytrap.com/forum/viewtopic.php?f=7&t=209&start=0.
CARROT CALLUS INTIATION MEDIUM FROM PITH TISSUE.CARROT CALLUS INTIATION MEDIUM FROM PITH TISSUE.
Product ID: C212
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, VITAMINS, & PLANT GROWTH REGULATORS REQUIRED TO INITIATE CARROT CALLUS
PLANT TISSUE CULTURE TESTED
C212
Carrot Callus Initiation
Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.21 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and plant growth
regulators required to initiate carrot callus from root tissue.
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture Tested
Plant species: Carrot (Daucus carota )
References
Establishment and Maintenance of Carrot Callus
Carrot [Daucus carota L. subsp. Sativus (Hoffm.)] is a cool season plant grown for its edible storage taproot. Since the discovery of somatic embryogenesis in carrot culture was made by Steward and Reinert, it has become a model system for investigating many biochemical, physiological, and genetic aspects of plant cell culture. This procedure will outline the method for establishing carrot callus cultures.
Materials Required
1. 10 glass or plastic petri dishes (100 mm in diameter)
2. 2 pairs of forceps and 2 scalpels
3. Sterile #2 cork borer and glass rod that fits it
4. 1000-ml beaker and 250-ml beaker
5. Waterproof marking pen and labels
6. 4 plastic slant racks to hold solid culture tubes
7. Analytical balance
8. Bunsen or ethanol burner
9. 500 ml of 20% Clorox solution supplemented with a few drops of Tween-20
10. 500 ml of sterile distilled water
11. 200 ml of 95% ethanol
12. Carrot Callus Initiation Medium, Product Number C212
13. Carrot Shoot Development Medium, Product Number C222
14. 1 or 2 healthy, undamaged, and regularly shaped carrot roots.
Procedures
1. Wipe down and turn on laminar flow hood 15 min before doing any work in the hood. Flame-sterilize instruments.
2. Clean carrot root by scrubbing under running tap water to remove any surface soil. Trim the carrot into 100-mm sections and place them in a 1000-ml beaker. Cover with 20% Clorox solution for approximately 20 min and then decant the Clorox solution. Rinse the explant three times in sterile distilled water covering the tissue with each rinse.
3. While carrot sections are being sterilized, prepare and dispense media into culture vessels according to protocols in the media preparation section. Place the culture vessels in racks and label them with respective treatments, medium, and date.
4. Transfer sterilized carrot slices to a sterile petri dish. Using a sterile cork borer, punch out 8-10 cylinders of tissue from the secondary phloem cambial region of the carrot slices. Do not punch out more than two cylinders of tissue at one time, as you may have difficulty in removing them from the cork borer. Using a sterile glass rod, push the cylinders from the cork borer into a sterile petri dish. Using a sharp, sterile scalpel, remove the ends of the tissue cylinder. Next cut the remaining portion of the cylinder into 2- to 3-mm-thick sections. Using a sterile petri plate on the balance, weigh each explant section individually and inoculate into the culture vessels containing the Carrot Callus Initiation Medium, placing one explant per tube. Repeat this procedure until all culture vessels containing the Carrot Callus Initiation medium have been inoculated. Use a different forcep for each explant and flame the forceps between transfers. Flame the lid of the culture tubes immediately after opening and before closing.
Callus should be removed from the primary explant after 45 days. The calli can be subcultured onto the same medium for further callus growth or it can be subcultured onto the Carrot Shoot Development medium for shoot initiation.
CARROT SHOOT DEVELOPMENT BASAL MEDIUMCARROT SHOOT DEVELOPMENT BASAL MEDIUM
Product ID: C222
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, VITAMINS, AND PGR'S REQUIRED TO INITIATE SHOOTS FROM CARROT CALLUS.
PLANT TISSUE CULTURE TESTED
C222 Carrot Shoot Development Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.21 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and plant growth
regulators required to initiate shoots from carrot callus.
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture Tested
Plant species: Carrot (Daucus carota )
References
CHEE & POOL C2d VITIS BASAL MEDIUMCHEE & POOL C2d VITIS BASAL MEDIUM
Product ID: C287
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY CHEE & POOL (1987).
PLANT TISSUE CULTURE TESTED
C287
Chee & Pool C2d Vitis
Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 4.49 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chee
and Pool (1987).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture Tested
Plant Species: Grape (Vitis spp)
This medium was developed to improve grape shoot multiplication. This formulation has
reduced levels of chloride, Iodide, and manganese compared to MS. Calcium chloride was
replaced by calcium nitrate, thus improving the quality of grave shoots.
References
Chee, R and RM Pool. 1987. Improved inorganic media constituents for in vitro shoot
multiplication of Vitis. Sci. Hort. 32: 85.
CHU N6 VITAMIN SOLUTION (1000x)CHU N6 VITAMIN SOLUTION (1000x)
Product ID: C149
Storage Temperature: 2 to 6 C
Description: CONTAINS THE VITAMINS DESCRIBED BY CHU (1975).USE AT A CONCENTRATION OF 1 mL/L OF MEDIUM TO ACHIEVE PROPER FINAL CONCENTRATION.
PLANT TISSUE CULTURE TESTED
C149
Chu N6 Vitamin Solution (1000x)
Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 1.0 mL/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Chu et al. (1975).
pH = 3.75 – 4.75
Application Notes
Plant Tissue Culture Tested
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659-
668.
CHU'S N6 BASAL MEDIUM w/ VITAMINS
Product ID: C149
Storage Temperature: 2 to 6 C
Description: CONTAINS THE VITAMINS DESCRIBED BY CHU (1975).USE AT A CONCENTRATION OF 1 mL/L OF MEDIUM TO ACHIEVE PROPER FINAL CONCENTRATION.
PLANT TISSUE CULTURE TESTED
C149
Chu N6 Vitamin Solution (1000x)
Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 1.0 mL/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Chu et al. (1975).
pH = 3.75 – 4.75
Application Notes
Plant Tissue Culture Tested
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659-
668.
CHU'S N6 BASAL MEDIUM w/ VITAMINSCHU'S N6 BASAL MEDIUM w/ VITAMINS
Product ID: C167
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY CHU (1975).
PLANT TISSUE CULTURE TESTED
C167
Chu N6 Basal Medium
w/ Vitamins
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.99 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chu
et al. (1975).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture Tested
Plant species: rice (Oryza sativa )
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus
from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4
+
is 7.0 mM compared to 20.6mM for MS.
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659-
668.
Product ID: C167
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY CHU (1975).
PLANT TISSUE CULTURE TESTED
C167
Chu N6 Basal Medium
w/ Vitamins
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.99 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chu
et al. (1975).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture Tested
Plant species: rice (Oryza sativa )
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus
from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4
+
is 7.0 mM compared to 20.6mM for MS.
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659-
668.
CHU'S N6 BASAL SALT MIXTURECHU'S N6 BASAL SALT MIXTURE
Product ID: C416
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY CHU (1975).
PLANT TISSUE CULTURE TESTED
C416
Chu N6 Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.98 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Chu et al. (1975).
pH = 3.75 – 4.75
Application Notes
Plant Tissue Culture Tested
Plant species: rice (Oryza sativa )
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus
from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4
+
is 7.0 mM compared to 20.6mM for MS.
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659-
668.
Product ID: C416
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY CHU (1975).
PLANT TISSUE CULTURE TESTED
C416
Chu N6 Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.98 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Chu et al. (1975).
pH = 3.75 – 4.75
Application NotesPlant Tissue Culture Tested
Plant species: rice (Oryza sativa )
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus
from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4
+
is 7.0 mM compared to 20.6mM for MS.
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659-
668.
DCR BASAL SALT MIXTUREDCR BASAL SALT MIXTURE
Product ID: D146
Storage Temperature: 2 to 6 C
Description: CONTAINS MACRO- AND MICRONUTRIENTS AS DESCRIBED BY GUPTA & DURZAN (1985).
PLANT TISSUE CULTURE TESTED
TARIFF CODE: 3105.90.0000
RISK & SAFETY: R: 8/36/37/38 S: 17/26/36
D146
DCR Basal Salt Mixture
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
1.64 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients as described by Gupta and Durzan. (1985).
pH = 3.5 – 4.5
Product ID: D146
Storage Temperature: 2 to 6 C
Description: CONTAINS MACRO- AND MICRONUTRIENTS AS DESCRIBED BY GUPTA & DURZAN (1985).
PLANT TISSUE CULTURE TESTED
TARIFF CODE: 3105.90.0000
RISK & SAFETY: R: 8/36/37/38 S: 17/26/36
D146
DCR Basal Salt Mixture
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
1.64 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients as described by Gupta and Durzan. (1985).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture Tested
Plant Species: Douglas Fir (Pseudotsuga spp.) and Sugar Pine (Pinus lambertiana)
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3- ions are supplied by Ca(NO3)2.
References
Gupta, PK and KJ Durzan. 1985. Shoot multiplication from mature Douglas-fir and sugar pine. Plant Cell Reports 4:177-179.
DKW BASAL MEDIUM, CONTAINS 10 g/L SUCROSEDKW BASAL MEDIUM, CONTAINS 10 g/L SUCROSE
Product ID: D191
Storage Temperature: 2 to 6 C
Description: W/O VITAMINS, CONTAINS MACRO- AND MICRONUTRIENTS AS DESCRIBED BY DRIVER & KUNIYUKI (1984) AND MCGRANAHAN ET AL. (1987).
The high calcium level in this medium may inhibit many gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum with this medium is not recommended as a clear, firm gel may not be obtained.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26/36
D191
DKW Basal Medium
Synonym:
DKW Basal Salts w/ Sucrose
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
15.22 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture Tested. Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, JA and AH Kuniyuki. 1984. HortSci 19:507-509.
McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
Product ID: D191
Storage Temperature: 2 to 6 C
Description: W/O VITAMINS, CONTAINS MACRO- AND MICRONUTRIENTS AS DESCRIBED BY DRIVER & KUNIYUKI (1984) AND MCGRANAHAN ET AL. (1987).
The high calcium level in this medium may inhibit many gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum with this medium is not recommended as a clear, firm gel may not be obtained.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26/36
D191
DKW Basal Medium
Synonym:
DKW Basal Salts w/ Sucrose
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
15.22 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture Tested. Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, JA and AH Kuniyuki. 1984. HortSci 19:507-509.
McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
DKW BASAL MEDIUM, CONTAINS 30 G/L SUCROSE.DKW BASAL MEDIUM, CONTAINS 30 G/L SUCROSE.
Product ID: D189
Storage Temperature: 2 to 6 C
Description: W/OUT VITAMINS; CONTAINS MACRO- AND MICRONUTRIENTS AS DESCRIBED BY DRIVER & KUNIYUKI (1984) AND MCGRANAHAN, et al. (1987)
The high calcium level in this medium may inhibit many gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum with this medium is not recommended as a clear, firm gel may not be obtained.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26/36
D189
DKW Basal Medium
Synonym:
DKW Basal Salts w/ Sucrose
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
35.22 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture
Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, JA and AH Kuniyuki. 1984. HortSci 19:507-509.
McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
DKW BASAL SALT MIXTUREDKW BASAL SALT MIXTURE
Product ID: D190
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY DRIVER & KUNIYUKI (1984) AND MCGRANAHAN, ET AL (1987).
The high calcium level in this medium may inhibit many gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum with this medium is not recommended as a clear, firm gel may not be obtained.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8/36/37/38 S: 17/26/36
D190
DKW Basal Salt Mixture
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
5.22 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture
Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, JA and AH Kuniyuki. 1984. HortSci 19:507-509.
McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
DKW MEDIUM W/ VITAMINS
DKW MEDIUM W/ VITAMINS
Product ID: D2470
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO-, MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY DRIVER & KUNIYUKI (1984) AND MCGRANAHAN, ET AL (1987).
The high calcium level in this medium may inhibit many gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum with this medium is not recommended as a clear, firm gel may not be obtained.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8/36/37/38 S: 17/26/36
D2470
DKW Basal Medium with Vitamins
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
5.32 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients and vitamins as described by Driver and Kuniyuki (1984) and corrected by McGranahan, et al. (1987).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture
Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, JA and AH Kuniyuki. 1984. HortSci 19:507-509.
McGranahan, GH, et al. 1962. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
RISK & SAFETY: R: 8/36/37/38 S: 17/26/36
D2470
DKW Basal Medium with Vitamins
Properties
Form:
Powder
Appearance:
White to Yellow Powder
Application:
Plant Tissue Culture
Solubility:
Water
Typical Working Concentration:
5.32 g/L
Storage Temp:
2 – 6° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes:
Contains the macro- and micronutrients and vitamins as described by Driver and Kuniyuki (1984) and corrected by McGranahan, et al. (1987).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture
Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, JA and AH Kuniyuki. 1984. HortSci 19:507-509.
McGranahan, GH, et al. 1962. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
ECONOMOU & READ BASAL MEDIUM
Product ID: E575
Storage Temperature: 2 to 6 C
Description:
Contains the macro- and micronutrients and vitamins as described by Economou & Read (1984)
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
ERIKSSON VITAMIN SOLUTION (1000x)
ERIKSSON VITAMIN SOLUTION (1000x)
Product ID: E330
Storage Temperature: 2 to 6 C
Description: CONTAINS THE VITAMINS DESCRIBED BY ERIKSON(1965).
USE A CONCENTRATION OF 1mL/L OF MEDIUM TO ACHIEVE PROPER FINAL CONCENTRATION
PLANT TISSUE CULTURE TESTED
STERILE
E330
Eriksson Vitamin Solution (1000x)
Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 1.0 mL/L of Medium
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Eriksson (1965).
pH = 3.75 – 4.75
Application Notes
Plant Tissue Culture Tested
References
Eriksson, T. 1965. Physiol. Plant 18:976-993.
EXS IIIa™ BASAL SALT MEDIUM WITH ADENINE
EXS IIIa™ BASAL SALT MEDIUM WITH ADENINE
Product ID: E333
Storage Temperature: 2 to 6 C
Description:
EXS IIIa™ is a proprietary plant cell culture medium that was originally developed for the culture of foliage plants such as Syngonium, Ficus, and Spathiphyllum.
PLANT TISSUE CULTURE TESTED
E333
EXS IIIa™ BASAL MEDIUM
Contains Adenine Hemisulfate
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.95 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Proprietary Formula
Contains myo-Inositol and Thiamine HCl
Does not contain carbohydrates or gelling agents
Application Notes
Plant Tissue Culture Tested
EXS IIIa™ is a proprietary plant cell culture medium that was originally developed for the culture
of foliage plants such as Syngonium , Ficus, and Spathiphyllum .
EXS IIIa™ has slightly less nitrogen and potassium compared to MS Medium, but higher
concentrations of calcium and magnesium. The reduced levels of nitrogen plus increased levels
of calcium and magnesium help support more productive growth in a wider range of plant
species.
References
EXS III™ BASAL MEDIUM W/OUT ADENINE
Product ID: E337
Storage Temperature: 2 to 6 C
Description:
EXS III™ is a proprietary plant cell culture medium that was originally developed for the culture of foliage plants such as Syngonium, Ficus, and Spathiphyllum.
PLANT TISSUE CULTURE TESTED
E337
EXS III™ BASAL MEDIUM
WITHOUT ADENINE HEMISULFATE
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.87 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Proprietary Formula
Contains myo-Inositol and Thiamine
Does not contain carbohydrates or gelling agents
pH = 4.25 – 5.25
Application Notes
Plant Tissue Culture Tested
EXS III™ is a proprietary plant cell culture medium that was originally developed for the culture
of foliage plants such as Syngonium , Ficus, and Spathiphyllum .
EXS III™ has slightly less nitrogen and potassium compared to MS Medium, but higher
concentrations of calcium and magnesium. The reduced levels of nitrogen plus increased levels
of calcium and magnesium help support more productive growth in a wider range of plant
species.
References
GAMBORG (PRL-4-DM) LONG BASAL MEDIUM
GAMBORG (PRL-4-DM) LONG BASAL MEDIUM
Product ID: G359
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1966).
PLANT TISSUE CULTURE TESTED
G359
Gamborg (PRL-4-DM) Long
Basal Medium
Synonym: Gamborg B-5 Long Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 2.64 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Gamborg (1966).
Application Notes
Plant Tissue Culture
References
Gamborg, OL, 1966. Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in
suspension cultures of plant cells. Can. J. Biochem. 44: 791-799.
GAMBORG B-5 BASAL MEDIUM
GAMBORG B-5 BASAL MEDIUM
Product ID: G398
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED
G398
Gamborg B-5 Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.21 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Gamborg, et al (1968).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture
Plant Species: Soybean (Glycine max )
This medium was developed for the initiation and growth of soybean cell suspensions. This
medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels
of potassium nitrate. Concentrations of NH4
+ over 2 mM inhibited cell growth.
Thiamine, an essential vitamin, is at a concentration 10x that of Linsmaier & Skoog Medium.
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
GAMBORG BASAL SALT MIXTURE
GAMBORG BASAL SALT MIXTURE
Product ID: G768
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED
G768
Gamborg B-5 Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.10 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Gamborg, et al
(1968).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture
Plant Species: Soybean (Glycine max )
This medium was developed for the initiation and growth of soybean cell suspensions. This
medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels
of potassium nitrate. Concentrations of NH4
+ over 2 mM inhibited cell growth.
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
GAMBORG VITAMIN POWDER (1000x)
GAMBORG VITAMIN POWDER (1000x)
Product ID: G249
Storage Temperature: 2 to 6 C
Description: CONTAINS THE VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED
G249
Gamborg Vitamin Powder (1000x)
Properties
Form: Powder
Appearance: White to Off-white powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration:
11.20 g/ 100 mL
1.0 mL/L of medium when reconstituted to volume indicated on the label.
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: 2 – 6° C
Other Notes: Contains the vitamins as described by Gamborg (1968).
Myo-Inositol may precipitate out of the prepared stock solution when cold;
warming with occasional agitation will redissolved the precipitated myo-
Inositol.
Application Notes
Plant Tissue Culture Tested
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
GAMBORG VITAMIN SOLUTION (1000x)
GAMBORG VITAMIN SOLUTION (1000x)
Product ID: G219
Storage Temperature: 2 to 6 C
Description: USE AT A CONCENTRATION OF 1 ML/L OF MEDIUM TO ACHIEVE THE PROPER FINAL CONCENTRATION
STERILE
PLANT TISSUE CULTURE TESTED
G219
Gamborg Vitamin Solution (1000x)
Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 1.0 mL/L of Medium
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Gamborg (1968).
Myo-Inositol may precipitate out of solution when cold; warming with
occasional agitation will redissolved the precipitate.
pH = 3.75 – 4.75
Application Notes
Plant Tissue Culture Tested
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient requirements of suspension cultures of
Product ID: E575
Storage Temperature: 2 to 6 C
Description:
Contains the macro- and micronutrients and vitamins as described by Economou & Read (1984)
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
ERIKSSON VITAMIN SOLUTION (1000x)ERIKSSON VITAMIN SOLUTION (1000x)
Product ID: E330
Storage Temperature: 2 to 6 C
Description: CONTAINS THE VITAMINS DESCRIBED BY ERIKSON(1965).
USE A CONCENTRATION OF 1mL/L OF MEDIUM TO ACHIEVE PROPER FINAL CONCENTRATION
PLANT TISSUE CULTURE TESTED
STERILE
E330
Eriksson Vitamin Solution (1000x)
Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 1.0 mL/L of Medium
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Eriksson (1965).
pH = 3.75 – 4.75
Application Notes
Plant Tissue Culture Tested
References
Eriksson, T. 1965. Physiol. Plant 18:976-993.
EXS IIIa™ BASAL SALT MEDIUM WITH ADENINEEXS IIIa™ BASAL SALT MEDIUM WITH ADENINE
Product ID: E333
Storage Temperature: 2 to 6 C
Description:
EXS IIIa™ is a proprietary plant cell culture medium that was originally developed for the culture of foliage plants such as Syngonium, Ficus, and Spathiphyllum.
PLANT TISSUE CULTURE TESTED
E333
EXS IIIa™ BASAL MEDIUM
Contains Adenine Hemisulfate
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.95 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Proprietary Formula
Contains myo-Inositol and Thiamine HCl
Does not contain carbohydrates or gelling agents
Application Notes
Plant Tissue Culture Tested
EXS IIIa™ is a proprietary plant cell culture medium that was originally developed for the culture
of foliage plants such as Syngonium , Ficus, and Spathiphyllum .
EXS IIIa™ has slightly less nitrogen and potassium compared to MS Medium, but higher
concentrations of calcium and magnesium. The reduced levels of nitrogen plus increased levels
of calcium and magnesium help support more productive growth in a wider range of plant
species.
References
EXS III™ BASAL MEDIUM W/OUT ADENINE
EXS III™ BASAL MEDIUM W/OUT ADENINE
Product ID: E337
Storage Temperature: 2 to 6 C
Description:
EXS III™ is a proprietary plant cell culture medium that was originally developed for the culture of foliage plants such as Syngonium, Ficus, and Spathiphyllum.
PLANT TISSUE CULTURE TESTED
E337
EXS III™ BASAL MEDIUM
WITHOUT ADENINE HEMISULFATE
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.87 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Proprietary Formula
Contains myo-Inositol and Thiamine
Does not contain carbohydrates or gelling agents
pH = 4.25 – 5.25
Application Notes
Plant Tissue Culture Tested
EXS III™ is a proprietary plant cell culture medium that was originally developed for the culture
of foliage plants such as Syngonium , Ficus, and Spathiphyllum .
EXS III™ has slightly less nitrogen and potassium compared to MS Medium, but higher
concentrations of calcium and magnesium. The reduced levels of nitrogen plus increased levels
of calcium and magnesium help support more productive growth in a wider range of plant
species.
References
GAMBORG (PRL-4-DM) LONG BASAL MEDIUMGAMBORG (PRL-4-DM) LONG BASAL MEDIUM
Product ID: G359
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1966).
PLANT TISSUE CULTURE TESTED
G359
Gamborg (PRL-4-DM) Long
Basal Medium
Synonym: Gamborg B-5 Long Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 2.64 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Gamborg (1966).
Application NotesPlant Tissue Culture
References
Gamborg, OL, 1966. Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in
suspension cultures of plant cells. Can. J. Biochem. 44: 791-799.
GAMBORG B-5 BASAL MEDIUMGAMBORG B-5 BASAL MEDIUM
Product ID: G398
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED
G398
Gamborg B-5 Basal Medium
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.21 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Gamborg, et al (1968).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture
Plant Species: Soybean (Glycine max )
This medium was developed for the initiation and growth of soybean cell suspensions. This
medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels
of potassium nitrate. Concentrations of NH4
+ over 2 mM inhibited cell growth.
Thiamine, an essential vitamin, is at a concentration 10x that of Linsmaier & Skoog Medium.
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
GAMBORG BASAL SALT MIXTURE GAMBORG BASAL SALT MIXTURE
Product ID: G768
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED
G768
Gamborg B-5 Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.10 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Gamborg, et al
(1968).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture
Plant Species: Soybean (Glycine max )
This medium was developed for the initiation and growth of soybean cell suspensions. This
medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels
of potassium nitrate. Concentrations of NH4
+ over 2 mM inhibited cell growth.
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
GAMBORG VITAMIN POWDER (1000x) GAMBORG VITAMIN POWDER (1000x)
Product ID: G249
Storage Temperature: 2 to 6 C
Description: CONTAINS THE VITAMINS AS DESCRIBED BY GAMBORG, ET AL. (1968).
PLANT TISSUE CULTURE TESTED
G249
Gamborg Vitamin Powder (1000x)
Properties
Form: Powder
Appearance: White to Off-white powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration:
11.20 g/ 100 mL
1.0 mL/L of medium when reconstituted to volume indicated on the label.
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: 2 – 6° C
Other Notes: Contains the vitamins as described by Gamborg (1968).
Myo-Inositol may precipitate out of the prepared stock solution when cold;
warming with occasional agitation will redissolved the precipitated myo-
Inositol.
Application NotesPlant Tissue Culture Tested
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
GAMBORG VITAMIN SOLUTION (1000x) GAMBORG VITAMIN SOLUTION (1000x)
Product ID: G219
Storage Temperature: 2 to 6 C
Description: USE AT A CONCENTRATION OF 1 ML/L OF MEDIUM TO ACHIEVE THE PROPER FINAL CONCENTRATION
STERILE
PLANT TISSUE CULTURE TESTED
G219
Gamborg Vitamin Solution (1000x)
Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 1.0 mL/L of Medium
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Gamborg (1968).
Myo-Inositol may precipitate out of solution when cold; warming with
occasional agitation will redissolved the precipitate.
pH = 3.75 – 4.75
Application NotesPlant Tissue Culture Tested
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient requirements of suspension cultures of
soybean root cells. Exp. Cell Research 50: 151-158.
HELLER BASAL SALT MIXTURE
HELLER BASAL SALT MIXTURE
Product ID: H393
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY HELLER (1953).
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
H393
Heller Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 1.64 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Heller (1953).
Application Notes
Plant Tissue Culture
References
Heller, R. 1953. Ann. Sci. Nat. Bot. Biol. Beg. 11th Ser. 14:1-223.
HELLER/WHITE MODIFIED BASAL SALT MIXTURE
HELLER/WHITE MODIFIED BASAL SALT MIXTURE
Product ID: H396
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY HELLER (1953) AND WHITE (1963). WITHOUT MOLYDENUM TRIOXIDE.
PLANT TISSUE CULTURE TESTED
H396
Heller/White Modified
Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 1.04 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Heller (1953) and White (1963).
Application Notes
Plant Tissue Culture
References
Heller, R. 1953. Ann. Sci. Nat. Bot. Biol. Beg. 11th Ser. 14:1-223.
White, PR. 1963. The Cultivation of Animal and Plant Cells, 2nd Edition, Ronald Press, NY, NY.
HOAGLAND MODIFIED BASAL SALT MIXTURE
HOAGLAND MODIFIED BASAL SALT MIXTURE
Product ID: H353
Storage Temperature: 2 to 6 C
Description:
CONTAINS FERROUS SULFATE AND THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY HOAGLAND AND ARNON (1950).
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
HOSTA INITIATION/MULTIPLICATION MEDIUM STAGE I/II MEDIUM
HOSTA INITIATION/MULTIPLICATION MEDIUM STAGE I/II MEDIUM
Product ID: H435
Storage Temperature: 2 to 6 C
Description: MODIFIED MURASHIGE & SKOOG W/(mg/L): 2.0 BA, 160 ADENINE HEMISULFATE, 500 CASEIN HYDROLYSATE, & .5 NAA
PLANT TISSUE CULTURE TESTED
H435
Hosta Initiation/ Multiplication Medium
Stage I/II Medium
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 43.39 g/L
Storage Temp: 2-6°
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and
Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965).
Also contains the supplements cited below.
Application Notes
Plant Tissue Culture Tested
Plant species: Hosta
Excellent stage I and II medium for Hosta cultures.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
HOSTA MULTIPLICATION MEDIUM STAGE II MEDIUM
HOSTA MULTIPLICATION MEDIUM STAGE II MEDIUM
Product ID: H436
Storage Temperature: 2 to 6 C
Description: MODIFIED M & S W/(mg/L): 0.1 6- BENZYLAMINOPURINE, 160 ADENINE HEMISULFATE, 500 CASEIN HYDROLYSATE, & .5 NAA
PLANT TISSUE CULTURE TESTED
H436
Hosta Multiplication Medium
Stage II Medium
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 43.39 g/L
Storage Temp: 2-6°
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and
Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965).
Also contains the supplements cited below.
Application Notes
Plant Tissue Culture Tested
Plant species: Hosta
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
HOSTA ROOTING MEDIUM STAGE III MEDIUM
HOSTA ROOTING MEDIUM STAGE III MEDIUM
Product ID: H437
Storage Temperature: 2 to 6 C
Description: MODIFIED MURASHIGE & SKOOG (1962) WITH (MG/L): 0.1 BA, 500.0 CASEIN HYDROLYSATE, AND 0.5 NAA
H437
Hosta Rooting Medium
Stage III Medium
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 43.23 g/L
Storage Temp: 2-6°
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and
Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965).
Also contains the supplements cited below.
Application Notes
Plant Tissue Culture Tested
Plant species: Hosta
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
KAO & MICHAYLUK BASAL SALT MIXTURE
KAO & MICHAYLUK BASAL SALT MIXTURE
Product ID: K413
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY KAO & MICHAYLUK (1975).
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
K413
Kao & Michayluk
Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.65 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Kao & Michayluk
(1975).
Application Notes
Plant Tissue Culture
Plant species: Vicia hajastana
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.
References
Kao, KN and MR Michayluk. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
KAO & MICHAYLUK MODIFIED BASAL MEDIUM
KAO & MICHAYLUK MODIFIED BASAL MEDIUM
Product ID: K427
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- & MICRONUTRIENTS, VITAMINS, & ORGANIC ACIDS AS DESCRIBED BY KAO & MICHAYLUK.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
K427
KAO & MICHAYLUK
MODIFIED BASAL MEDIUM
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.9 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and organic supplements as described by Kao and Michayluk (1975).
Application Notes
Plant Tissue Culture Tested
Plant species: Vicia hajastana
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.
References
Kao, KN and MR Michayluk. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
KAO & MICHAYLUK VITAMIN SOLUTION (100x)
KAO & MICHAYLUK VITAMIN SOLUTION (100x)
Product ID: K421
Storage Temperature: 2 to 6 C
Description: USE AT A CONCENTRATION OF 10 mL/L OF MEDIUM TO ACHIEVE THE PROPER FINAL CONCENTRATION.
STERILE: PASSES USP STERILITY TESTING
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26-36
K421
Kao & Michayluk Vitamin Solution (100x)
Properties
Form: Liquid
Appearance: Clear, Colorless to Slight Yellow Tint Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 10.0 mL/L of Medium
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Kao and Michayluk (1975).
Application Notes
Plant Tissue Culture Tested
References
Kao, KN and MR Michayluk. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
LINSMAIER & SKOOG BASAL MEDIUM
LINSMAIER & SKOOG BASAL MEDIUM
Product ID: L689
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- & MICRONUTRIENTS, & VITAMINS DESCRIBED BY LINSMAIER & SKOOG (1964).
This medium may be sold as Murashige & Skoog (MS) Medium with Minimal Organics (MSMO) by some media suppliers.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
L689
Linsmaier & Skoog
Basal Medium
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 4.43 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Linsmaier and Skoog (1965).
pH = 3.5 – 4.5
Application Notes
Plant Tissue Culture Tested
Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog. Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L. Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7 g/L AGAR
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7 g/L AGAR
Product ID: L467
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY LINSMAIER & SKOOG (1965).
This medium may be sold as Murashige & Skoog (MS) Medium with Minimal Organics (MSMO) by some media suppliers.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26-36
L467
Linsmaier & Skoog Basal Medium
w/ 30 g/L Sucrose and 7 g/L Agar
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 41.43 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Linsmaier and Skoog (1965).
pH = 4.25 – 5.25
Application Notes
Plant Tissue Culture Tested
Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog. Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L. Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7g/L AGAR, pH ADJUSTED & BUFFERED
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7g/L AGAR, pH ADJUSTED & BUFFERED
Product ID: L452
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO-& MICRONUTRIENTS, & VITAMINS AS DESCRIBED BY LINSMAIER & SKOOG (1965)
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26-36
L452
Linsmaier & Skoog Modified Basal Medium
w/ 30 g/L Sucrose & 7 g/L Agar
pH Adjusted and Buffered
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 42.53 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Linsmaier and Skoog (1965).
Application Notes
Plant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuations during media preparation and culture.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
HELLER BASAL SALT MIXTUREHELLER BASAL SALT MIXTURE
Product ID: H393
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY HELLER (1953).
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
H393
Heller Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 1.64 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Heller (1953).
Application NotesPlant Tissue Culture
References
Heller, R. 1953. Ann. Sci. Nat. Bot. Biol. Beg. 11th Ser. 14:1-223.
HELLER/WHITE MODIFIED BASAL SALT MIXTUREHELLER/WHITE MODIFIED BASAL SALT MIXTURE
Product ID: H396
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY HELLER (1953) AND WHITE (1963). WITHOUT MOLYDENUM TRIOXIDE.
PLANT TISSUE CULTURE TESTED
H396
Heller/White Modified
Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 1.04 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Heller (1953) and White (1963).
Application NotesPlant Tissue Culture
References
Heller, R. 1953. Ann. Sci. Nat. Bot. Biol. Beg. 11th Ser. 14:1-223.
White, PR. 1963. The Cultivation of Animal and Plant Cells, 2nd Edition, Ronald Press, NY, NY.
HOAGLAND MODIFIED BASAL SALT MIXTUREHOAGLAND MODIFIED BASAL SALT MIXTURE
Product ID: H353
Storage Temperature: 2 to 6 C
Description:
CONTAINS FERROUS SULFATE AND THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY HOAGLAND AND ARNON (1950).
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
HOSTA INITIATION/MULTIPLICATION MEDIUM STAGE I/II MEDIUMHOSTA INITIATION/MULTIPLICATION MEDIUM STAGE I/II MEDIUM
Product ID: H435
Storage Temperature: 2 to 6 C
Description: MODIFIED MURASHIGE & SKOOG W/(mg/L): 2.0 BA, 160 ADENINE HEMISULFATE, 500 CASEIN HYDROLYSATE, & .5 NAA
PLANT TISSUE CULTURE TESTED
H435
Hosta Initiation/ Multiplication Medium
Stage I/II Medium
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 43.39 g/L
Storage Temp: 2-6°
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and
Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965).
Also contains the supplements cited below.
Application NotesPlant Tissue Culture Tested
Plant species: Hosta
Excellent stage I and II medium for Hosta cultures.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
HOSTA MULTIPLICATION MEDIUM STAGE II MEDIUMHOSTA MULTIPLICATION MEDIUM STAGE II MEDIUM
Product ID: H436
Storage Temperature: 2 to 6 C
Description: MODIFIED M & S W/(mg/L): 0.1 6- BENZYLAMINOPURINE, 160 ADENINE HEMISULFATE, 500 CASEIN HYDROLYSATE, & .5 NAA
PLANT TISSUE CULTURE TESTED
H436
Hosta Multiplication Medium
Stage II Medium
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 43.39 g/L
Storage Temp: 2-6°
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and
Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965).
Also contains the supplements cited below.
Application NotesPlant Tissue Culture Tested
Plant species: Hosta
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
HOSTA ROOTING MEDIUM STAGE III MEDIUM HOSTA ROOTING MEDIUM STAGE III MEDIUM
Product ID: H437
Storage Temperature: 2 to 6 C
Description: MODIFIED MURASHIGE & SKOOG (1962) WITH (MG/L): 0.1 BA, 500.0 CASEIN HYDROLYSATE, AND 0.5 NAA
H437
Hosta Rooting Medium
Stage III Medium
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 43.23 g/L
Storage Temp: 2-6°
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and
Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965).
Also contains the supplements cited below.
Application NotesPlant Tissue Culture Tested
Plant species: Hosta
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
KAO & MICHAYLUK BASAL SALT MIXTURE KAO & MICHAYLUK BASAL SALT MIXTURE
Product ID: K413
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS AS DESCRIBED BY KAO & MICHAYLUK (1975).
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
K413
Kao & Michayluk
Basal Salt Mixture
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.65 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Kao & Michayluk
(1975).
Application NotesPlant Tissue Culture
Plant species: Vicia hajastana
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.
References
Kao, KN and MR Michayluk. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
KAO & MICHAYLUK MODIFIED BASAL MEDIUM KAO & MICHAYLUK MODIFIED BASAL MEDIUM
Product ID: K427
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- & MICRONUTRIENTS, VITAMINS, & ORGANIC ACIDS AS DESCRIBED BY KAO & MICHAYLUK.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
K427
KAO & MICHAYLUK
MODIFIED BASAL MEDIUM
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 3.9 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and organic supplements as described by Kao and Michayluk (1975).
Application NotesPlant Tissue Culture Tested
Plant species: Vicia hajastana
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.
References
Kao, KN and MR Michayluk. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
KAO & MICHAYLUK VITAMIN SOLUTION (100x) KAO & MICHAYLUK VITAMIN SOLUTION (100x)
Product ID: K421
Storage Temperature: 2 to 6 C
Description: USE AT A CONCENTRATION OF 10 mL/L OF MEDIUM TO ACHIEVE THE PROPER FINAL CONCENTRATION.
STERILE: PASSES USP STERILITY TESTING
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26-36
K421
Kao & Michayluk Vitamin Solution (100x)
Properties
Form: Liquid
Appearance: Clear, Colorless to Slight Yellow Tint Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working
Concentration: 10.0 mL/L of Medium
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution: N/A
Other Notes: Contains the vitamins as described by Kao and Michayluk (1975).
Application NotesPlant Tissue Culture Tested
References
Kao, KN and MR Michayluk. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
LINSMAIER & SKOOG BASAL MEDIUMLINSMAIER & SKOOG BASAL MEDIUM
Product ID: L689
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- & MICRONUTRIENTS, & VITAMINS DESCRIBED BY LINSMAIER & SKOOG (1964).
This medium may be sold as Murashige & Skoog (MS) Medium with Minimal Organics (MSMO) by some media suppliers.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 8-36/37/38 S: 17-26-36
L689
Linsmaier & Skoog
Basal Medium
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 4.43 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Linsmaier and Skoog (1965).
pH = 3.5 – 4.5
Application NotesPlant Tissue Culture Tested
Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog. Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L. Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7 g/L AGARLINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7 g/L AGAR
Product ID: L467
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO- AND MICRONUTRIENTS, AND VITAMINS AS DESCRIBED BY LINSMAIER & SKOOG (1965).
This medium may be sold as Murashige & Skoog (MS) Medium with Minimal Organics (MSMO) by some media suppliers.
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26-36
L467
Linsmaier & Skoog Basal Medium
w/ 30 g/L Sucrose and 7 g/L Agar
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 41.43 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Linsmaier and Skoog (1965).
pH = 4.25 – 5.25
Application NotesPlant Tissue Culture Tested
Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog. Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L. Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7g/L AGAR, pH ADJUSTED & BUFFEREDLINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7g/L AGAR, pH ADJUSTED & BUFFERED
Product ID: L452
Storage Temperature: 2 to 6 C
Description: CONTAINS THE MACRO-& MICRONUTRIENTS, & VITAMINS AS DESCRIBED BY LINSMAIER & SKOOG (1965)
PLANT TISSUE CULTURE TESTED
RISK & SAFETY: R: 36/37/38 S: 26-36
L452
Linsmaier & Skoog Modified Basal Medium
w/ 30 g/L Sucrose & 7 g/L Agar
pH Adjusted and Buffered
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 42.53 g/L
Storage Temp: 2 – 6° C
Storage Temp of
Stock Solution:
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by
Linsmaier and Skoog (1965).
Application NotesPlant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuations during media preparation and culture.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
